Title: A novel sialic acid-specific lectin from Phaseolus coccineus seeds with potent antineoplastic and antifungal activities
Authors: Chen, Jing ×
Liu, Bo
Ji, Na
Zhou, Jing
Van Damme, Els
Balzarini, Jan
Chen, Fang
Bao, Jin-Ku #
Issue Date: Apr-2009
Publisher: G. Fischer
Series Title: Phytomedicine vol:16 issue:4 pages:352-360
Abstract: A novel lectin (PCL) with specificity towards sialic acid was purified from Phaseolus coccineus L. (P. multiflorus willd) seeds using ion exchange chromatography on CM and DEAE-Sepharose, and gel filtration on Sephacryl S-200 column. PCL was a homodimer consisting of 29,831.265Da subunits as determined by gel filtration and MS. Also, PCL was a non-metaloprotein and its N-terminal 20-amino acid sequence, MASSALLSLALFLVLLTKAN, was determined. Subsequently, MTT method, cell morphological analysis and LDH activity-based cytotoxicity assays demonstrated that PCL was highly cytotoxic to L929 cells and induced apoptosis in a dose-dependent manner. Using caspase inhibitors, a typical caspase-dependent pathway was confirmed. PCL also showed remarkable antifungal activity towards some plant pathogenic fungi. Furthermore, when sialic acid-specific activity was fully inhibited, cytotoxicity and antifungal activity were abruptly decreased, respectively, suggesting a significant correlation between sialic acid-specific site and its bi-functional bioactivities.
ISSN: 0944-7113
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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