Title: Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside
Authors: Nauwelaerts, Koen ×
Vastmans, Karen
Froeyen, Mathy
Kempeneers, Veerle
Rozenski, Jef
Rosemeyer, Helmut
Van Aerschot, Arthur
Busson, Roger
Lacey, Jeffrey C
Efimtseva, Ekaterina
Mikhailov, Sergey
Lescrinier, Eveline
Herdewijn, Piet #
Issue Date: Dec-2003
Series Title: Nucleic acids research vol:31 issue:23 pages:6758-69
Abstract: A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.
ISSN: 0305-1048
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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