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Title: The intron-containing genome of the lytic Pseudomonas phage LUZ24 resembles the temperate phage PaP3
Authors: Ceyssens, Pieter-Jan ×
Hertveldt, Kirsten
Ackermann, Hans-W
Noben, Jean-Paul
Demeke, Mekonnen
Volckaert, Guido
Lavigne, Rob #
Issue Date: Aug-2008
Publisher: Academic press inc elsevier science
Series Title: Virology vol:377 issue:2 pages:233-238
Abstract: The virulent Pseudomonas aeruginosa bacteriophage LUZ24 (45,625 bp) was isolated from hospital sewage. It belongs to the family of the Podoviridae, and carries a bidirectionally transcribed dsDNA genome delineated by two direct terminal repeats of 184 bp. In vitro transcriptional analysis identified seven sigma(70) promoters, revealing a bias towards stronger promoter strength in the late genomic region. Reverse transcription demonstrated in vivo splicing of a 668 bp Group I intron embedded inside the DNA polymerase gene. Using mass spectrometry, nine structural proteins were identified as part of the phage particle. The lytic characteristics of LUZ24 are evaluated against its genomic content, which displays an overall 71% sequence similarity to the temperate phage PaP3. (C) 2008 Elsevier Inc. All rights reserved.
URI: 
ISSN: 0042-6822
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Molecular Microbiology and Biotechnology Section - miscellaneous
× corresponding author
# (joint) last author

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