Title: Structural basis for inhibition of Aspergillus niger xylanase by triticum aestivum xylanase inhibitor-I
Authors: Sansen, Stefaan ×
De Ranter, Camiel
Gebruers, Kurt
Brijs, Kristof
Courtin, Christophe
Delcour, Jan
Rabijns, Anja #
Issue Date: Aug-2004
Series Title: Journal of Biological Chemistry vol:279 issue:34 pages:36022-36028
Abstract: Plants developed a diverse battery of defense mechanisms in response to continual challenges by a broad spectrum of pathogenic microorganisms. Their defense arsenal includes inhibitors of cell wall-degrading enzymes, which hinder a possible invasion and colonization by antagonists. The structure of Triticum aestivum xylanase inhibitor-I (TAXI-I), a first member of potent TAXI-type inhibitors of fungal and bacterial family 11 xylanases, has been determined to 1.7-A resolution. Surprisingly, TAXI-I displays structural homology with the pepsin-like family of aspartic proteases but is proteolytically nonfunctional, because one or more residues of the essential catalytical triad are absent. The structure of the TAXI-I.Aspergillus niger xylanase I complex, at a resolution of 1.8 A, illustrates the ability of tight binding and inhibition with subnanomolar affinity and indicates the importance of the C-terminal end for the differences in xylanase specificity among different TAXI-type inhibitors.
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biocrystallography
Centre for Food and Microbial Technology
Administration Rectorial Services - miscellaneous
× corresponding author
# (joint) last author

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