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Title: Chiral capillary electrophoretic method for quantification of apomorphine
Authors: Ha, Pham Thi Thanh ×
Van Schepdael, Ann
Van Vaeck, Laurence
Augustijns, Patrick
Hoogmartens, Jos #
Issue Date: Sep-2004
Series Title: Journal of Chromatography A vol:1049 issue:1-2 pages:195-203
Abstract: A new method for chiral determination of apomorphine enantiomers was developed and validated. Seven different neutral and charged cyclodextrins were tested for enantioselectivity on R,S-apomorphine. Sulfobutylether-beta-cyclodextrin was found to offer the best resolution, but with this system, four peaks were detected from a solution of the two enantiomers, which was suggested to be the result of different forms of the complex between the selector and apomorphine. A complexation constant was estimated for a complex of 1:1 ratio for the second and the fourth peak, whereas the other two peaks were fitted to a model ratio of 1:2 (analyte-selector). To avoid this phenomenon, hydroxypropyl-beta-cyclodextrin was then chosen as the chiral selector. An optimisation study was performed on three factors: concentration of the chiral selector, pH of the buffer, and applied voltage. Optimum conditions were: 14 mM of hydroxypropyl-beta-cyclodextrin, pH 3.0, and 16 kV. UV detection was at 200 nm. The method was validated at the chosen conditions, offering a limit of detection of 0.2 microM and a limit of quantification of 0.5 microM. The validated method was applied for the determination of R,S-apomorphine in a transport study with an in vitro cell culture model of the intestinal mucosa (Caco-2).
ISSN: 0021-9673
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
Drug Delivery and Disposition
× corresponding author
# (joint) last author

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