Characterisation of reversed-phase liquid chromatographic columns by chromatographic tests. Evaluation of 36 test parameters: repeatability, reproducibility and correlation
Visky, Dóra × Vander Heyden, Yvan Iványi, Tímea Baten, Peggy De Beer, Jacques Kovács, Zsuzsanna Noszál, Béla Roets, Eugene Massart, Désiré L Hoogmartens, Jos #
Journal of Chromatography A vol:977 issue:1 pages:39-58
The European Pharmacopoeia (Ph. Eur.) or other official compendia give only a general description of the stationary phase in the description of a liquid chromatographic method. Therefore the selection of a column giving suitable selectivity presents difficulties. Earlier, a test procedure was proposed that allows measurement of a number of parameters which are reported to be representative for stationary phase characteristics. This paper describes how the test procedure was applied on 69 RP-LC C18 columns. Chromatographic parameters obtained as test results were evaluated, and their repeatability, reproducibility and correlation were examined.