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Title: Probe-independent and direct quantification of insulin mRNA and growth hormone mRNA in enriched cell preparations
Authors: Van Lommel, Leentje ×
Janssens, Kristel
Quintens, Roel
Tsukamoto, Katsura
Vander Mierde, Dirk
Lemaire, Katleen
Denef, Carl
Jonas, Jean-Christophe
Martens, Geert
Pipeleers, Daniel
Schuit, Frans #
Issue Date: Dec-2006
Series Title: Diabetes vol:55 issue:12 pages:3214-3220
Abstract: Task division in multicellular organisms ensures that differentiated cell types produce cell-specific proteins that fulfill tasks for the whole organism. In some cases, the encoded mRNA species is so abundant that it represents a sizeable fraction of total mRNA in the cell. In this study, we have used a probe- and primer-free technique to quantify such abundant mRNA species in order to assess regulatory effects of in vitro and in vivo conditions. As a first example, we were able to quantify the regulation of proinsulin mRNA abundance in beta-cells by food intake or by the glucose concentration in tissue culture. The second example of application of this technique is the effect of corticosteroids on growth hormone mRNA in enriched somatrotrophs. It is anticipated that other examples exist in which measurement of very abundant mRNAs in dedicated cells will help to understand biological processes, monitor disease states, or assist biotechnological manufacturing procedures.
URI: 
ISSN: 0012-1797
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Pharmacology Section (-)
Gene Expression Unit
× corresponding author
# (joint) last author

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