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Title: Human skeletal muscle atrophy in amyotrophic lateral sclerosis reveals a reduction in Akt and an increase in atrogin-1
Authors: Léger, Bertrand ×
Vergani, Lodovica
Sorarù, Gianni
Hespel, Peter
Derave, Wim
Gobelet, Charles
D'Ascenzio, Carla
Angelini, Corrado
Russell, Aaron P #
Issue Date: Mar-2006
Publisher: The Federation of American Societies for Experimental Biology
Series Title: FASEB Journal vol:20 issue:3 pages:583-5
Abstract: The molecular mechanisms influencing muscle atrophy in humans are poorly understood. Atrogin-1 and MuRF1, two ubiquitin E3-ligases, mediate rodent and cell muscle atrophy and are suggested to be regulated by an Akt/Forkhead (FKHR) signaling pathway. Here we investigated the expression of atrogin-1, MuRF1, and the activity of Akt and its catabolic (FKHR and FKHRL1) and anabolic (p70(s6k) and GSK-3beta) targets in human skeletal muscle atrophy. The muscle atrophy model used was amyotrophic lateral sclerosis (ALS). All measurements were performed in biopsies from 22 ALS patients and 16 healthy controls as well as in G93A ALS mice. ALS patients had a significant increase in atrogin-1 mRNA and protein content, which was associated with a decrease in Akt activity. There was no difference in the mRNA and protein content of FKHR, FKHRL1, p70(s6k), and GSK-3beta. Similar observations were made in the G93A ALS mice. Human skeletal muscle atrophy, as seen in the ALS model, is associated with an increase in atrogin-1 and a decrease in Akt. The transcriptional regulation of human atrogin-1 may be controlled by an Akt-mediated transcription factor other than FKHR or via another signaling pathway.
URI: 
ISSN: 0892-6638
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Exercise Physiology Research Group
× corresponding author
# (joint) last author

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