Ten female participants performed work at a video display terminal (VDT) station over a whole working day. Subjective local muscular fatigue was evaluated by means of the Category Ratio 10 scale. Electromyographic activity of the upper right and left trapezius was measured. A comparison was made between five participants who had previous complaints and 5 participants who reported no musculoskeletal problems in the shoulder-neck region. The subjective scores for the shoulder differed significantly between the two groups, being higher for the group with complaints. Both groups showed a decrease in discomfort after the lunch break. The activity of the trapezius increased significantly for both groups, in a more pronounced way for the group with disorders. Although it is found in literature that VDT work is a task with very low static loads, it seems from this study that the EMG activity increase can be an indication of muscle fatigue: more effort was required to accomplish the same VDT task at the end of the day.