Title: Protein profiling based on two-dimensional difference gel electrophoresis
Authors: Van den Bergh, Gert
Arckens, Lut
Issue Date: 2008
Publisher: Methods in Molecular Biology
Host Document: Genomics Protocols edition:Volume: 439 pages:211-224
Article number: 15
Abstract: The recent introduction of two-dimensional fluorescent difference gel electrophoresis has enabled the large screening of differential protein expression levels with higher confidence and greater sensitivity than using the classical two-dimensional electrophoresis approach. With this technology, multiple protein samples can be labeled with up to three fluorescent dyes. These labeled protein samples are mixed and applied on the same two-dimensional electrophoresis gel, subsequently scanned, and analyzed by specialized software. The possibility to run two or more protein samples on a single gel as well as the introduction of an internal standard on each gel drastically reduces the gel-to-gel variability and results in higher levels of certainty with regard to the differential character of the expressed proteins.
ISBN: 978-1-58829-871-3
Publication status: published
KU Leuven publication type: IHb
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous

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