Title: Identification of new regional marker proteins to map mouse brain by 2-D difference gel electrophoresis screening
Authors: Jacobs, Sandy ×
Van de Plas, Babs
Van der Gucht, Estel
Clerens, Stefan
Cnops, Lieselotte
Van den Bergh, Gert
Arckens, Lut #
Issue Date: Apr-2008
Publisher: Wiley-v c h verlag gmbh
Series Title: Electrophoresis vol:29 issue:7 pages:1518-1524
Abstract: To screen for new region-specific protein markers we compared the proteome maps of the primary visual and somatosensory areas V1 and S1 in mouse brain using 2-D difference gel electrophoresis (2-D DIGE). Twenty-three protein spots showed a statistically significant difference in expression level between V1 and S1, with 52% appearing more abundantly in V1. Twenty-six proteins were mass spectrometrically identified in 22 spots. To assess the validity of this list of potential areal markers generated by 2-1) DIGE, the effective areaspecific distribution profile of creatine kinase brain subtype (CKB), a protein with a clearly higher expression level in S1, was monitored with in situ hybridization. The mRNA expression profile of CKB displayed a clear area-specific distribution, which allowed demarcation of S1 and its topographical borders with neighboring neocortical areas. This proteomic study demonstrates the innovative application of 2-D DIGE and MS to select new regional markers for neuroscience research.
ISSN: 0173-0835
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Research Group Neuroplasticity and Neuroproteomics (-)
Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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