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Title: Experimental chlamydial keratitis in rabbits. Correlation with chlamydia infected McCoy tissue culture cells
Authors: Abu el-Asrar, A M ×
Maudgal, P C
Emarah, M H
Missotten, Luc #
Issue Date: Jan-1989
Series Title: Documenta ophthalmologica. Advances in ophthalmology vol:69 issue:4 pages:353-69
Abstract: Rabbit corneas were inoculated three times at weekly intervals with the agent of chlamydia trachomatis using the scratch method. Specimens of the corneal epithelium were obtained using the replica technique on the 1st, 2nd, 3rd and 4th day after each inoculation and at two weeks after the last inoculation. The development of chlamydial inclusions and the inflammatory cell response were monitored using Giemsa stain, acridine orange stain and direct immunofluorescent technique. Primary inoculation produced mild clinical disease associated cytologically with polymorphonuclear leucocytic cellular inflammatory response. Repeated inoculations produced more severe disease associated clinically with pannus formation and cytologically with the presence of lymphocytes and Leber cells in addition to polymorphonuclear leucocytes. Halberstaedter Prowazek inclusion bodies were detected in all the specimens. Additional intracytoplasmic and intranuclear inclusions of different morphological appearances were present. The cytological findings detected in the corneal epithelium of rabbits were correlated with the findings in McCoy tissue culture cells inoculated with chlamydia trachomatis.
ISSN: 0012-4486
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Alumni, Faculty of Medicine
× corresponding author
# (joint) last author

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