We evaluated colour contrast thresholds in patients with unilateral functional amblyopia. A computer-controlled colour vision test was used to determine colour contrast thresholds along a blue-yellow tritan axis. Prior to the threshold measurement, a heterochromatic flicker brightness test was performed to ensure isoluminance between the test stimulus and the background for every subject individually. Luminance contrast thresholds were also measured using the same computer-based system. Twenty amblyopic patients were tested. All showed normal tritan colour contrast thresholds both for their amblyopic eye and non-amblyopic fellow eye. Luminance contrast thresholds were elevated in all 6 refractive amblyopic eyes, in 4 of 9 strabismic eyes and in 2 of 5 mixed amblyopic eyes. Colour contrast thresholds are normal in functional amblyopia (with central fixation). Whenever elevated tritan colour contrast thresholds are found in patients with a decreased visual acuity, other causes of visual impairment are to be evaluated.