Title: gamma-glutamyl transpeptidase in transgenic tobacco plants. Cellular localization, processing, and biochemical properties
Authors: Storozhenko, S ×
Belles-Boix, E
Babiychuk, E
Herouart, D
Davey, Mark
Slooten, L
Van Montagu, M
Inze, D
Kushnir, S #
Issue Date: Mar-2002
Publisher: Amer soc plant biologists
Series Title: Plant physiology vol:128 issue:3 pages:1109-1119
Abstract: gamma-Glutamyl transpeptidase (gamma-GT) is a ubiquitous enzyme that catalyzes the first step of glutathione (GSH) degradation in the gamma-glutamyl cycle in mammals. A cDNA encoding an Arabidopsis homolog for gamma-GT was overexpressed in tobacco (Nicotiona tabacum) plants. A high level of the membrane-bound gamma-GT activity was localized outside the cell in transgenic plants. The overproduced enzyme was characterized by a high affinity to GSH and was cleaved post-translationally in two unequal subunits. Thus, Arabidopsis gamma-GT is similar to the mammalian enzymes in enzymatic properties, post-translational processing, and cellular localization, suggesting analogous biological functions as a key enzyme in the catabolism of GSH.
ISSN: 0032-0889
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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