Title: Maturation of stretch-induced contractile activity and its muscarinic regulation in isolated whole bladder strips from rat
Authors: Gevaert, Thomas ×
Owsianik, Grzegorz
Hutchings, Graham
Everaerts, Wouter
Nilius, Bernd
De Ridder, Dirk #
Issue Date: Jun-2010
Series Title: Neurourology and Urodynamics vol:29 issue:5 pages:789-796
Abstract: AIM: Besides the establishment of neural reflex pathways, developmental changes in local bladder properties probably also contribute to the onset of mature voiding reflexes. Here we explored the behavior of stretch-induced contractile activity (SIC) and its muscarinic regulation in neonatal and adult rat bladders. METHODS: SIC was studied in bladder strips from D0, D7, D28 rat pups and adult rats (15 weeks). The responses to a non-selective [Carbachol (CA), 10(-8.5)-10(-6) M] and an M(2)-selective muscarinic agonist [arecaidine but-2-ynyl ester tosylate (ABET), 10(-9.5)-10(-7) M] were studied. The expression of M(2) and M(3) mRNA was investigated using quantitative PCR in whole bladders. The response of SIC to KCl (50 mM) and to the non-adrenergic non-cholinergic (NANC) drugs Substance P (1 microM) and alpha,beta-Methyleneadenosine 5'-triphosphate lithium salt (MATP) (1 microM) were also studied. RESULTS: We found evidence for an enhanced response to the muscarinic agonists CA and ABET in neonatal bladders. This might be due to the onset of a direct contractile role for M(2), given the moderate M(2)-properties of ABET and the absence of ABET-effects on adult bladder strips. Further data showed an increased expression of both M(2) and M(3) at the mRNA level and a changed response of SIC to NANC drugs in neonatal bladder. CONCLUSIONS: This study reveals a changed response of SIC to muscarinic and NANC drugs in neonatal rat bladder together with changes at the muscarinic mRNA level, which might all contribute to a better insight in the role of SIC in the onset of mature voiding. Neurourol. Urodynam. (c) 2008 Wiley-Liss, Inc.
ISSN: 0733-2467
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Urology Section (-)
Laboratory of Ion Channel Research
Section Woman - Miscellaneous (-)
Physiology Section (-)
Screening, Diagnostics and Biomarkers (-)
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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