Title: Alpha-oligodeoxynucleotides as inhibitors of hiv reverse-transcriptase
Authors: Pauwels, R ×
Debyser, Zeger
Balzarini, Jan
Baba, M
Desmyter, Jan
Rayner, B
Morvan, F
Imbach, Jl
De Clercq, Erik #
Issue Date: 1989
Publisher: Marcel dekker inc
Series Title: Nucleosides & nucleotides vol:8 issue:5-6 pages:995-1000
Abstract: A number of oligodeoxynucleotides containing thymine bases linked to the deoxyribose in the -configuration have been synthesized. In view of the possible activity of -origomers as antisense compounds against HIV replication, the effects of -thymidylate oligomers (-dTn, n=1-1-16) in a poly (A)-directed HIV reverse transcriptase (RT) reaction were investigated. No priming activity could be detected with the -dT oligomers at concentrations up to 100 μM. However, in the presence of the natural β-dT12-18 primer, 50% inhibition of RT activity was achieved at a concentration of about 1 μM with the -dT oligomers containing ≥ 10 monomer units. The kinetics of RT inhibition by the -decamer was found to be competitive with respect to the natural primer, β-dT10.
ISSN: 0732-8311
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Virology and Gene Therapy
Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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