ICAAC edition:41th location:Chicago date:16-19 December 2001
BACKGROUND: The aim of the present study was to determine the prevalence of antibiotic resistance in CSF isolates and to describe the most common serotypes during 1997-2000. METHODS: 237 CSF isolates were collected in Belgian hospitals and mailed to the national reference center. Strains were typed using Neufelds reaction with 46 sera obtained from the Statens Seruminstitut (Copenhagen). MICs of penicillin, cefotaxime, cefepime, meropenem and vancomycin were determined on blood-supplemented Mueller-Hinton agar plates with the E test (AB Biodisk, Solna, Sweden). RESULTS: The 10 most common serogroups representing 76% of the isolates were 14, 6, 9, 19, 23, 18, 4, 10, 8 and 12 (in order of frequency). 36% of strains were isolated in children < 5 years old. In this age group the number of serogroups was more limited and 81.4% are included in the 7-valent conjugate vaccine. The MIC values are shown in the table [table: see text]. Reduced susceptibility to penicillin (MIC > 0.06 mcg/ml) was observed in 30 (12.6%) of 237 strains. Only 5 were fully resistant (MIC > 1 mcg/ml) and belonged to serogroups 14 (2 x), 23 (2 x) and 21 (1 x). CONCLUSIONS: CSF strains of S. pneumoniae with reduced susceptibility to penicillin are emerging in Belgium, but resistance to third generation cephalosporins remains rare.