Title: Furin and proprotein convertase 7 (PC7) lymphoma PC endogenously expressed in rat liver can be resolved into distinct post-Golgi compartments
Authors: Wouters, S
Leruth, M
Decroly, E
Vandenbranden, M
Creemers, John
Van De Loo, Jwhp
Ruysschaert, Jm
Courtoy, Pj #
Issue Date: 1998
Series Title: Biochemical Journal vol:336 issue:Part 2 pages:311-316
Abstract: The intracellular compartmentalization in rat liver of the membrane-associated convertases furin and proprotein convertase 7 (PC7)/lymphoma PC (LPC) was investigated by analytical subcellular fractionation. In control animals, both enzymes were found to localize in fractions depleted of endoplasmic reticulum, cis-Golgi and lysosomal markers, but to co-distribute with the Golgi marker galactosyltransferase and the trans-Golgi network (TGN) marker TGN38. After over-loading Golgi-derived vesicles with very-low-density lipoproteins (VLDL) by feeding rats with ethanol, the distribution of PC7/LPC was shifted markedly towards lower densities, in contrast with those of furin and the TGN marker. This provides support for the TGN localization of endogenously expressed furin and indicates that, at steady state, a considerable proportion of PC7/LPC may be associated with vesicles derived from the TGN.
ISSN: 0264-6021
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Genetics Section (-)
Department of Human Genetics - miscellaneous
# (joint) last author

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