Title: Proprotein Processing Activity And Cleavage Site Selectivity of The Kex2-Like Endoprotease Pace4
Authors: Creemers, John ×
Groot Kormelink, P J
Roebroek, Anton
Nakayama, K
Van de Ven, Willem #
Issue Date: 1993
Series Title: FEBS Letters vol:336 issue:1 pages:65-69
Abstract: Proprotein processing activity of the Kex2-like mammalian endoprotease PACE4 and its cleavage selectivity for sites with basic amino acid residues were determined. Using a recombinant vaccinia virus-based expression system, PACE4 was expressed in pig kidney PK(15) cells and, like two other Kex2-like endoproteases furin and PC6A, shown to correctly process the precursor of von Willebrand factor (pro-vWF). Furthermore, characteristics of the cleavage site selectivity of PACE4 were compared to those of furin and PC6A using the vWF cleavage site mutants vWFR-1G, vWFK-2A, and vWFR-4A as substrates. Cleavage site selectivity of PACE4 and PC6A appeared to be similar but they differed from that of furin.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Associated Laboratories - miscellaneous (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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