Title: The effect of a Sertoli cell-selective knockout of the androgen receptor on testicular gene expression in prepubertal mice
Authors: Denolet, Evi
De Gendt, Karel
Allemeersch, Joke
Engelen, Kristof
Marchal, Kathleen
Van Hummelen, Paul
Tan K. A., L
Sharpe R., M
Saunders P. T., K
Swinnen, Johan
Verhoeven, Guido # ×
Issue Date: Feb-2006
Series Title: Molecular Endocrinology vol:20 issue:2 pages:321-334
Abstract: To unravel the molecular mechanisms mediating the effects of androgens on spermatogenesis, testicular gene expression was compared in mice with Sertoli cell-selective androgen receptor knockout (SCARKO) and littermate controls on postnatal d 10. Microarray analysis identified 692 genes with significant differences in expression. Of these, 28 appeared to be down-regulated and 12 up-regulated at least 2-fold in SCARKOs compared with controls. For nine of the more than 2-fold down-regulated genes, androgen regulation was confirmed by treatment of wild-type mice with an antiandrogen (flutamide). Some of them were previously described to be androgen regulated or essential for spermatogenesis. Serine-type protease inhibitors were markedly overrepresented in this down-regulated subgroup. A time study (d 8-20), followed by cluster analysis, allowed identification of distinct expression patterns of differentially expressed genes. Three genes with a pattern closely resembling that of Pem, a prototypical androgen-regulated gene expressed in Sertoli cells, were selected for confirmation by quantitative RT-PCR and additional analysis. The data confirm that the SCARKO model allows identification of novel androgen-regulated genes in the testis. Moreover, they suggest that protease inhibitors and other proteins related to tubular restructuring and cell junction dynamics may be controlled in part by androgens.
Description: \emph{Molecular Endocrinology}, vol. 20, no. 2, Feb. 2006
ISSN: 0888-8809
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Faculty of Medicine - miscellaneous
Clinical and Experimental Endocrinology
Nucleomics Core
Laboratory of Lipid Metabolism and Cancer (+)
× corresponding author
# (joint) last author

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