Title: Reaction of the type III iodothyronine deiodinase with the affinity label N-bromoacetyl-triiodothyronine
Authors: Schoenmakers, Chh ×
Pigmans, Igaj
Kaptein, E
Darras, Veerle
Visser, Tj #
Issue Date: 28-Sep-1993
Series Title: Febs Letters vol:335 issue:1 pages:104-108
Abstract: The type III iodothyronine deiodinase (ID-III) catalyzes the inner ring deiodination and, thus, the inactivation of the thyroid hormones T-4 and T-3. ID-III activity in rat brain, rat placenta and embryonic chicken liver is inhibited by the affinity label N-bromoacetyl-T-3 (BrAcT3) with an affinity similar to that of T-3. Reaction of rat brain and placenta microsomes with BrAc[I-125]T-3 resulted in the extensive labeling of a 32 kDa protein (p32). However, p32 was also prominently labeled in fetal rat liver microsomes which have no ID-III activity. Labeling of p32 was not influenced by 100 mu M substrate analogs or inhibitors of ID-III, some of which completely inhibit ID-III activity at 1 mu M. BrAc[I-125]T-3 labeling of embryonic chicken liver microsomes did not reveal p32 or another protein possibly related to ID-III, In contrast to previous suggestions, it is unlikely that p32 represents ID-III or a subunit thereof.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Schoenmakers et al._1993_FEBS Letters_vol335_p104-108.pdf Published 1771KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science