Title: Relative activity of endoxylanases towards water-extractable and water-unextractable arabinoxylans in wheat bran
Authors: Courtin, Christophe ×
Delcour, Jan #
Issue Date: May-2001
Publisher: Academic press ltd
Series Title: Journal of cereal science vol:33 issue:3 pages:301-312
Abstract: Differences in endo-beta -1,4-xylanase functionality result from the different ability of the enzymes to convert water-unextractable arabinoxylan (WU-AX) into solubilised AX (S-AX) and to degrade water-extractable arabinoxylan (WE-AX) and S-AX to lower molecular weight. These respective endoxylanse activities and their relative proportions were deternubed for two endoxylanses from Bacillus subtilis and Aspergillus aculeatus, according to a generally applicable procedure. Isolated wheat WE-AX and WU-AX containing wheat squeege starch prepared and incubated with different levels of endoxylanases. The supernatants were analysed for reducing xylose (RX) formed and WU-AX solublised. Molecular weights of the products formed were assessed. Relstive activities differed widely for the two endoxylanses. Based on the results and additional enzyme-substrate binding experiments, mechanisms possibly accounting for these differences are discussed. (C) 2001 Academic Press.
ISSN: 0733-5210
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Maes 2004 J Cereal Sci 39 181-186.pdf Published 126KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science