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Title: A comparative immunohistochemical study of human corneotrabecular tissue
Authors: Foets, Beatrijs ×
van den Oord, Joost
Engelmann, K
Missotten, Luc #
Issue Date: Jul-1992
Series Title: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie vol:230 issue:3 pages:269-74
Abstract: Using in situ immunohistochemical techniques and a broad panel of antibodies directed against intermediate filament proteins, vascular endothelial markers, neuroectodermal/neuroendocrine markers, and monoclonal antibodies raised against human corneal endothelial cells (HCECs), a comparative phenotypical analysis was performed on HCECs, keratocytes, trabecular cells, and cells lining the canal of Schlemm. The coexpression of cytokeratins and neurofilaments by HCECs argues in favor of a neuroectodermal origin, which is further supported by the fact that they stain positive for neuron-specific enolase (NSE) and that they express neural cell adhesion molecules (N-CAM) at their surface. The expression of NSE and N-CAM also applies to the trabecular cells. The cells lining the canal of Schlemm were found to share many immunophenotypical features with vascular endothelial cells (i.e., factor-VIII-related antigen and BMA-120), rather than with HCECs. This was further supported by the reactivity of two monoclonal antibodies (i.e., 9.3 E and 5.52 H) that were raised against HCECs, and which labelled vascular endothelium and cells lining Schlemm's canal.
ISSN: 0721-832X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Cell & Tissue Research
Faculty of Medicine - miscellaneous
Research Group Ophthalmology
Alumni, Faculty of Medicine
× corresponding author
# (joint) last author

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