Title: Binding of a monoclonal antibody positively correlates with bioactivity of the F4 fimbrial adhesin FaeG associated with post-weaning diarrhoea in piglets
Authors: Verdonck, F ×
Snoeck, V
Goddeeris, Bruno
Cox, E #
Issue Date: Nov-2004
Publisher: Elsevier science bv
Series Title: Journal of Immunological Methods vol:294 issue:1-2 pages:81-88
Abstract: Piglets are susceptible to F4 (K88)(+) enterotoxigenic Escherichia coli (ETEC)-induced neonatal and post-weaning diarrhoea. The F4 fimbriae are composed of some minor subunits and the major subunit FaeG that also constitutes the adhesin. Parenteral vaccination of sows with an F4-containing vaccine protects the suckling piglets against neonatal F4(+) ETEC-induced diarrhoea, but no commercial (mucosal) vaccine exists against F4(+) ETEC-induced weaning diarrhoea. To develop a vaccine, a bioactive F4-receptor (F4R) binding FaeG molecule is required that binds to the F4R following oral immunization and induces a FaeG-specific immune response. The present study reports the altered binding of the FaeG-specific monoclonal antibody IMM01 with bioactive versus non-bioactive F4 fimbrial adhesin FaeG. The correlation of altered IMM01 binding with altered FaeG bioactivity permits the use of an IMM01-based ELISA as a fast, specific and sensitive in vitro selection for potent F4 or (recombinant) FaeG antigen formulations, useful in an F4(+) ETEC vaccine. (C) 2004 Elsevier B.V. All rights reserved.
ISSN: 0022-1759
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
× corresponding author
# (joint) last author

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