Title: The genome and structural Proteome of YuA, a new Pseudomonas aeruginosa phage resembling M6
Authors: Ceyssens, Pieter-Jan ×
Mesyanzhinov, Vadim
Sykilinda, Nina
Briers, Yves
Roucourt, Bart
Lavigne, Rob
Robben, Johan
Domashin, Artem
Miroshnikov, Konstantin
Volckaert, Guido
Hertveldt, Kirsten #
Issue Date: Feb-2008
Publisher: Amer soc microbiology
Series Title: Journal of bacteriology vol:190 issue:4 pages:1429-1435
Abstract: Pseudomonas aeruginosa phage YuA (Siphoviridae) was isolated from a pond near Moscow, Russia. It has an elongated head, encapsulating a circularly permuted genome of 58,663 bp, and a flexible, noncontractile tail, which is terminally and subterminally decorated with short fibers. The YuA genome is neither Mu- nor lambda-like and encodes 78 gene products that cluster in three major regions involved in (i) DNA metabolism and replication, (ii) host interaction, and (iii) phage particle formation and host lysis. At the protein level, YuA displays significant homology with phages M6, phi JL001, 73, B3, DMS3, and D3112. Eighteen YuA proteins were identified as part of the phage particle by mass spectrometry analysis. Five different bacteria] promoters were experimentally identified using a promoter trap assay, three of which have a sigma(54)-specific binding site and regulate transcription in the genome region involved in phage particle formation and host lysis. The dependency of these promoters on the host sigma(54) factor was confirmed by analysis of an rpoN mutant strain of P. aeruginosa PAO1. At the DNA level, YuA is 91% identical to the recently (July 2007) annotated phage M6 of the Lindberg typing set. Despite this level of DNA homology throughout the genome, both phages combined have 15 unique genes that do not occur in the other phage. The genome organization of both phages differs substantially from those of the other known Pseudomonas-infecting Siphoviridae, delineating them as a distinct genus within this family.
ISSN: 0021-9193
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Biochemistry, Molecular and Structural Biology Section
Laboratory of Glycobiology and Developmental Genetics (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
published.pdfmain article Published 446KbAdobe PDFView/Open


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science