Evidence for fine-scale genetic structure and estuarine colonisation in a potential high gene flow marine goby (Pomatoschistus minutus)

Pampoulie, C; Gysels, ES; Maes, Gregory; Hellemans, Bart; Leentjes, V; Jones, AG; Volckaert, Filip

doi:10.1038/sj.hdy.6800438

Evidence for fine-scale genetic structure and estuarine colonisation in a potential high gene flow marine goby (Pomatoschistus minutus)

Author:

Pampoulie, C

Gysels, ES ; Maes, Gregory ; Hellemans, Bart ; Leentjes, V ; Jones, AG ; Volckaert, Filip

Keywords:

allele shift, allozymes, gene flow, microsatellites, north atlantic ocean, sand goby, bass dicentrarchus-labrax, cod gadus-morhua, induced sand transport, population-structure, allozyme variation, sexual selection, microsatellite variation, anadromous fishes, fresh-water, nuclear-dna, Science & Technology, Life Sciences & Biomedicine, Ecology, Evolutionary Biology, Genetics & Heredity, Environmental Sciences & Ecology, North Atlantic Ocean, COD GADUS-MORHUA, INDUCED SAND TRANSPORT, POPULATION-STRUCTURE, SEXUAL SELECTION, MICROSATELLITE VARIATION, ALLOZYME VARIATION, NUCLEAR-DNA, FRESH-WATER, SEA, DIFFERENTIATION, Alleles, Animals, Gene Frequency, Gene Transfer, Horizontal, Genotype, Microsatellite Repeats, North Sea, Perciformes, Polymorphism, Genetic, Selection, Genetic, Sequence Analysis, DNA, 0603 Evolutionary Biology, 0604 Genetics, 3104 Evolutionary biology, 3105 Genetics

Abstract:

Marine fish seem to experience evolutionary processes that are expected to produce genetically homogeneous populations. We have assessed genetic diversity and differentiation in 15 samples of the sand goby Pomatoschistus minutus (Pallas, 1770) (Gobiidae, Teleostei) from four major habitats within the Southern Bight of the North Sea, using seven microsatellite and 13 allozyme loci. Despite its high dispersal potential, microsatellite loci revealed a moderate level of differentiation (overall F-ST = 0.026; overall R-ST = 0.058). Both hierarchical analysis of molecular variance and multivariate analysis revealed significant differentiation (P<0.01) between estuarine, coastal and marine samples with microsatellites, but not with allozymes. Comparison among the different estimators of differentiation (F-ST and R-ST) pointed to possible historical events and contemporary habitat fragmentation. Samples were assigned to two breeding units in the estuary and coastal region. Despite this classification, there were indications of a complex and dynamic spatiotemporal structure, which is, most likely, determined by historical events and local oceanic currents.