Title: Disruption of nasopharyngeal epithelium by pneumococci is density-linked
Authors: Lagrou, Katrien ×
Peetermans, Willy
Verhaegen, Jan
Jorissen, Mark
Van Eldere, Johan #
Issue Date: Mar-2003
Series Title: European Journal of Clinical Investigation vol:33 issue:4 pages:340-5
Abstract: BACKGROUND: The aim of this project was to study the influence of pneumococci on nasopharyngeal epithelial integrity as a function of time and pneumococcal density. MATERIALS AND METHODS: Cell layers of an in vitro model of human nasopharyngeal epithelium were inoculated with different pneumococcal strains. The transepithelial electrical resistance (TEER), a measure of the integrity of the cell layers, and the pneumococcal concentration in the apical fluid on the epithelial cells were measured at different times after inoculation. RESULTS: Pneumococci caused a decrease in the TEER when a density of 1 x 107 CFU mL-1 was reached. The growth rate of pneumococci in our in vitro model differed between the strains tested and, for the same strain, between in vitro culture on the epithelial cells and broth culture. Differences in timing of the onset of decrease in the TEER between strains were the result of differences in growth rate on the epithelial cells. Antibiotic-induced lysis of pneumococci caused an immediate decrease in the TEER of the cell layers. CONCLUSION: Pneumococci cause a decrease in the TEER at a density of 1 x 107 CFU mL-1. Our hypothesis is that this decrease in the TEER is the result of quorum-induced lysis of the pneumococci.
ISSN: 0014-2972
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Research Group Experimental Oto-rhino-laryngology
Laboratory for Clinical Infectious and Inflammatory Disorders
Laboratory of Clinical Bacteriology and Mycology
Laboratory for Experimental and Clinical Microbiology (-)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science