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Title: Adalimumab induces apoptosis of human monocytes: a comparative study with infliximab and etanercept
Authors: Shen, Chong ×
Assche, G V
Colpaert, Steven
Maerten, P
Geboes, Karel
Rutgeerts, Paul
Ceuppens, Jan #
Issue Date: Feb-2005
Series Title: Alimentary Pharmacology & Therapeutics vol:21 issue:3 pages:251-8
Abstract: BACKGROUND: Adalimumab, a fully human monoclonal antibody to tumour necrosis factor, was recently introduced for therapy of Crohn's disease. AIM: Since induction of apoptosis of inflammatory cells is thought to be an important mechanism of action of the antitumour necrosis factor monoclonal antibody infliximab, we studied the induction of apoptosis of activated peripheral blood monocytes by adalimumab. METHOD: Apoptosis was analysed at the levels of the cell membrane, mitochondria and DNA by flow cytometry. RESULTS: We found that both adalimumab and infliximab induced apoptosis in cultured monocytes, while etanercept did not. Apoptosis induction was caspase-dependent and detectable already after 2 h. The production of interleukin-10 and interleukin-12 by monocytes was down-regulated significantly by adalimumab and infliximab but not by etanercept, while levels of soluble tumour necrosis factor in monocyte cultures were down-regulated by all three reagents. CONCLUSIONS: These data show that both adalimumab and infliximab affect monocyte cytokine production and induce apoptosis of activated monocytes. Our findings will have to be further correlated to therapeutic efficacy of these antitumour necrosis factor reagents.
ISSN: 0269-2813
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical Residents Medicine
Translational Cell & Tissue Research
Translational Research in GastroIntestinal Disorders
Laboratory of Clinical Immunology
× corresponding author
# (joint) last author

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