Title: The role of hemocytes, serine protease inhibitors and pathogen-associated patterns in prophenoloxidase activation in the desert locust, Schistocerca gregaria
Authors: Franssens, Vanessa * ×
Simonet, Gert *
Breugelmans, Bert *
Van Soest, Sofie
van Hoef, Vincent
Vanden Broeck, Jozef #
Issue Date: Feb-2008
Series Title: Peptides vol:29 issue:2 pages:235-41
Abstract: The prophenoloxidase-activating system is an important component of the innate immune response of insects, involved in wound healing and melanotic encapsulation. In this paper we show that in the desert locust, Schistocerca gregaria, hemocytes, challenged with microbial elicitors, are indispensable for the limited proteolytic activation of prophenoloxidase (proPO) in plasma. In addition, we assessed the influence of serine protease inhibitors on the induction of PO-activity in plasma. While soybean Bowman-Birk inhibitor (SBBI) inhibited the PO activation by laminarin-treated hemocytes, the endogenous pacifastin-related inhibitors, SGPI-1 (S. gregaria pacifastin-related inhibitor-1) and SGPI-2 did not affect the PO-activity under similar conditions. On the other hand, real-time PCR analysis revealed that the transcripts, encoding SGPI-1-3, were more abundant in the fat body of immune challenged animals, as compared to control animals.
ISSN: 0196-9781
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Department of Biology - miscellaneous
Experimental Cardiology
Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
* (joint) first author
× corresponding author
# (joint) last author

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