Title: Purification and characterization of an insulin-related peptide in the desert locust, Schistocerca gregaria: immunolocalization, cDNA cloning, transcript profiling and interaction with neuroparsin
Authors: Badisco, Liesbeth ×
Claeys, Ilse
Van Hiel, Boris
Clynen, Elke
Huybrechts, Jurgen
Vandersmissen, Tim
Van Soest, Sofie
Vanden Bosch, Luc
Simonet, Gert
Vanden Broeck, Jozef #
Issue Date: Mar-2008
Publisher: Journal of Endocrinology (Ltd. by Guarantee)
Series Title: Journal of molecular endocrinology vol:40 issue:3 pages:137-50
Abstract: Members of the insulin superfamily are not restricted to vertebrates, but have also been identified in invertebrate species. In the current report, we present the characterization of Scg-insulin-related peptide (IRP), an insulin-related peptide in the desert locust, Schistocerca gregaria. This peptide was isolated from corpora cardiaca (CC) extracts by means of a high-performance liquid chromatography (HPLC)-based purification strategy. Subsequent cloning and sequencing of the corresponding cDNA revealed that the encoded Scg-IRP precursor displays the structural organization that is typical for members of the insulin superfamily. Moreover, immunocytochemistry on brain tissue sections demonstrated the presence of Scg-IRP in median neurosecretory cells of the pars intercerebralis and their projections towards the storage part of the CC. Quantitative real-time RT-PCR studies revealed the presence of Scg-IRP transcripts in a variety of tissues, including nervous tissue and fat body. Furthermore, these transcripts showed a tissue- and phase-dependent, temporal regulation during the reproductive cycle of adult males and females. Finally, we demonstrated that Scg-IRP interacts in vitro with a recombinant neuroparsin, a locust protein displaying sequence similarity with vertebrate IGF binding proteins.
ISSN: 0952-5041
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Research Group Molecular Developmental Physiology and Signal Transduction (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Badisco et al._2008_J Mol Endocrinol_vol41_p137-150.pdf Published 680KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science