Title: Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network
Authors: Liljedahl, M
Maeda, Y
Colanzi, A
Ayala, I
Van Lint, Johan
Malhotra, V # ×
Issue Date: 23-Feb-2001
Publisher: MIT Press
Series Title: Cell vol:104 issue:3 pages:409-420
Abstract: When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface.
ISSN: 0092-8674
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Laboratory of Protein Phosphorylation and Proteomics
× corresponding author
# (joint) last author

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