Title: Post-transcriptional modification mapping in the Clostridium actobutylicum 16S rRNA by massaspectrometry and reversed transcriptase assays
Authors: Emmerechts, Gert ×
Barbé, Sofie
Herdewijn, Piet
Anné, Jozef
Rozenski, Jef #
Issue Date: 2007
Publisher: Oxford University Press
Series Title: Nucleic acids research vol:35 pages:3494-3503
Abstract: Post-transcriptional modifications in ribosomal RNA are believed to fine-tune the RNA functions. The present study describes the characterization of the post-transcriptional modifications in Clostridium acetobutylicum 16S rRNA,
using high-pressure liquid chromatography (HPLC) coupled to electrospray ionization mass spectrometry and reverse transcriptase assays. The combination of these techniques allowed the identification of eleven modified nucleosides,
which were mapped onto the rRNA sequence. The C. acetobutylicum modification map is similar to that of Escherichia coli, with the majority of the modifications near functionally important sites in the rRNA. Although, in general, the number of modifications in rRNA is smaller than in tRNA, the conservation of the modification sites seems to
indicate that the post-transcriptional modifications in 16S rRNA provide a necessary prerequisite for the ribosomal function.
ISSN: 0305-1048
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Laboratory of Molecular Bacteriology (Rega Institute)
× corresponding author
# (joint) last author

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