Title: Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
Authors: Adelfinskaya, Olga
Terrazas, Montserat
Froeyen, Mathy
Marlière, Philippe
Nauwelaerts, Koen
Herdewijn, Piet # ×
Issue Date: 2007
Publisher: Oxford University Press
Series Title: Nucleic acids research vol:35 pages:5060-5072
Abstract: Some selected amino acids, in particular L-aspartic acid (L-Asp) and L-histidine (L-His), can function as leaving group during polymerase-catalyzed incorporation of deoxyadenosine monophosphate (dAMP) in DNA. Although L-Asp-dAMP and L-His-dAMP bind, most probably, in a different way in the active site of the enzyme, aspartic acid and histidine can be considered as mimics of the
pyrophosphate moiety of deoxyadenosine triphosphate.
L-Aspartic acid is more efficient than D-aspartic acid as leaving group. Such P-N conjugates of amino acids and deoxynucleotides provide a novel experimental ground for diversifying nucleic acid metabolism in the field of synthetic biology
ISSN: 0305-1048
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
× corresponding author
# (joint) last author

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