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Title: Conditional deletion of Smad1 and Smad5 in somatic cells of male and female gonads leads to metastatic tumor development in mice
Authors: Pangas, Stephanie A ×
Li, Xiaohui
Umans, Lieve
Zwijsen, An
Huylebroeck, Danny
Gutierrez, Carolina
Wang, Degang
Martin, James F
Jamin, Soazik P
Behringer, Richard R
Robertson, Elizabeth J
Matzuk, Martin M #
Issue Date: Jan-2008
Publisher: Amer soc microbiology
Series Title: Molecular and cellular biology vol:28 issue:1 pages:248-257
Abstract: The transforming growth factor beta (TGF beta) family has critical roles in the regulation of fertility. In addition, the pathogenesis of some human cancers is attributed to misregulation of TGF beta function and SMAD2 or SMAD4 mutations. There are limited mouse models for the BMP signaling SMADs (BR-SMADs) 1, 5, and 8 because of embryonic lethality and suspected genetic redundancy. Using tissue-specific ablation in mice, we deleted the BR-SMADs from somatic cells of ovaries and testes. Single conditional knockouts for Smad1 or Smad5 or mice homozygous null for Smad8 are viable and fertile. Female double Smad1 Smad5 and triple Smad1 Smad5 Smad8 conditional knockout mice become infertile and develop metastatic granulosa cell tumors. Male double Smad1 Smad5 conditional knockout mice are fertile but demonstrate metastatic testicular tumor development. Microarray analysis indicated significant alterations in expression of genes related to the TGF beta pathway, as well as genes involved in infertility and extracellular matrix production. These data strongly implicate the BR-SMADs as part of a critical developmental pathway in ovaries and testis that, when disrupted, leads to malignant transformation.
ISSN: 0270-7306
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Biology (Celgen) (-)
Embryo and Stemcells (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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