Title: Visualization of membrane rafts using a perylene monoimide derivative and fluorescence lifetime Imaging
Authors: Margineanu, Anca ×
Hotta, Jun-Ichi
Van der Auweraer, Mark
Ameloot, Marcel
Stefan, Alina
Beljonne, David
Engelborghs, Yves
Herrmann, Andreas
Muellen, Klaus
De Schryver, Frans
Hofkens, Johan #
Issue Date: Oct-2007
Publisher: Biophysical Society
Series Title: Biophysical Journal vol:93 issue:8 pages:2877-2891
Abstract: A new membrane probe, based on the perylene imide chromophore, with excellent photophysical properties ( high absorption coefficient, quantum yield (QY) approximate to 1, high photostability) and excited in the visible domain is proposed for the study of membrane rafts. Visualization of separation between the liquid-ordered (Lo) and the liquid-disordered (Ld) phases can be achieved in artificial membranes by fluorescence lifetime imaging due to the different decay times of the membrane probe in the two phases. Rafts on micrometer-scale in cell membranes due to cellular activation can also be observed by this method. The decay time of the dye in the Lo phase is higher than in organic solvents where its QY is 1. This allows proposing a ( possible general) mechanism for the decay time increase in the Lo phase, based on the local field effects of the surrounding molecules. For other fluorophores with QY < 1, the suggested mechanism could also contribute, in addition to effects reducing the non-radiative decay pathways, to an increase of the fluorescence decay time in the Lo phase.
ISSN: 0006-3495
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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