Title: Single molecule fluorescence spectroscopy of pH sensitive oligonucleotide switches
Authors: Kolaric, Branko ×
SLIWA, Michel
Brucale, Marco
Vallee, Renaud A. L
Zuccheri, Giampaolo
Samori, Bruno
Hofkens, Johan
De Schryver, Frans #
Issue Date: Jun-2007
Publisher: Royal Society of Chemistry
Series Title: Photochemical & Photobiological Sciences vol:6 issue:6 pages:614-618
Abstract: Several authors demonstrated that an oligonucleotide based pH-sensitive construct can act as a switch between an open and a closed state by changing the pH. To validate this process, specially designed fluorescence dye-quencher substituted oligonucleotide constructs were developed to probe the switching between these two states. This paper reports on bulk and single molecule fluorescence investigations of a duplex-triplex pH sensitive oligonucleotide switch. On the bulk level, only a partial quenching of the fluorescence is observed, similarly to what is observed for other published switches and is supposed to be due to intermolecular interactions between oligonucleotide strands. On the single molecule level, each DNA-based nanometric construct shows a complete switching. These observations suggest the tendency of the DNA construct to associate at high concentration.
ISSN: 1474-905X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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