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Title: Ultrafast excited-state dynamics of the photoswitchable protein dronpa
Authors: Fron, Eduard ×
Flors Ong, Cristina
Schweitzer, Gerd
Habuchi, Satoshi
Mizuno, Hideaki
Ando, Ryoko
De Schryver, Frans
Miyawaki, Atsushi
Hofkens, Johan #
Issue Date: Apr-2007
Publisher: American Chemical Society
Series Title: Journal of the American Chemical Society vol:129 issue:16 pages:4870-+
Abstract: Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A(2)). We have used femtosecond transient absorption spectroscopy to determine the kinetics of the first step of the photoconversion from the dark to the bright form, which we have measured as 4 ps. The 2-fold isotope effect on the kinetics of this process shows that excited-state proton transfer (ESPT) is involved in this step. It was also demonstrated that the acid-induced protonated form A(1) and the photoconverted protonated form A(2) are two distinct spectroscopic species.
ISSN: 0002-7863
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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