Title: Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase
Authors: Jabafi, Ilham ×
Selisko, Barbara
Coutard, Bruno
De Palma, Armando M
Neyts, Johan
Egloff, Marie-Pierre
Grisel, Sacha
Dalle, Karen
Campanacci, Valerie
Spinelli, Silvia
Cambillau, Christian
Canard, Bruno
Gruez, Arnaud #
Issue Date: Jun-2007
Series Title: Acta Crystallographica F, Structural Biology and Crystallization Communications Online vol:63 pages:495-498
Abstract: The Picornaviridae virus family contains a large number of human pathogens such as poliovirus, hepatitis A virus and rhinoviruses. Amongst the viruses belonging to the genus Enterovirus, several serotypes of coxsackievirus coexist for which neither vaccine nor therapy is available. Coxsackievirus B3 is involved in the development of acute myocarditis and dilated cardiomyopathy and is thought to be an important cause of sudden death in young adults. Here, the first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported. Standard crystallization methods yielded crystals that were poorly suited to X-ray diffraction studies, with one axis being completely disordered. Crystallization was improved by testing crystallization solutions from commercial screens as additives. This approach yielded crystals that diffracted to 2.1 angstrom resolution and that were suitable for structure determination.
ISSN: 1744-3091
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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