Title: Complexation of lipofectarnine and cholesterol-modified DNA sequences studied by single-molecule fluorescence techniques
Authors: Margineanu, Anca ×
De Feyter, Steven
Melnikov, Sergey
Marchand, Damien
Van Aerschot, Arthur
Herdewijn, Piet
Habuchi, Satoshi
De Schryver, Frans
Hofkens, Johan #
Issue Date: Nov-2007
Publisher: Amer chemical soc
Series Title: Biomacromolecules vol:8 issue:11 pages:3382-3392
Abstract: Lipoplex formation for normal and cholesterol-modified oligonucleotides is investigated by fluorescence correlation spectroscopy (FCS). To overcome the problems related to the fitting of autocorrelation curves when fluorescence bursts are present, the baseline fluorescence levels and the fluorescence bursts in the same trace were separately analyzed. This approach was not previously used in FCS studies of lipoplexes and allowed a more detailed characterization of this heterogeneous system. From the baseline levels, the number of free/bound DNA molecules and the presence of tens to hundreds of nanometer-sized lipoplexes were estimated using various mathematical models. Analysis of the fluorescent bursts provided an indication about the sizes of the lipoplexes, the number of DNA molecules in these aggregates, and the relative amount of lipids in each aggregate. An explanation for the higher transfection efficiency previously reported for one of the cholesterol-modified oligonucleotide compounds was found in relation to the formation of large size lipoplexes.
ISSN: 1525-7797
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry (Rega Institute)
Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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