Title: Synthesis and invitro evaluation of [leu13]porcine motilin fragments
Authors: Macielag, Mj ×
Peeters, Tl
Konteatis, Zd
Florance, Jr
Depoortere, Inge
Lessor, Ra
Bare, La
Cheng, Ys
Galdes, A #
Issue Date: May-1992
Publisher: Pergamon-elsevier science ltd
Series Title: Peptides vol:13 issue:3 pages:565-569
Abstract: Several peptide fragments representing N-terminal, C-terminal, and internal sequences of [Leu13]porcine motilin ([Leu13]pMOT) were synthesized using Fmoc solid phase methodology. Peptides were assayed for motilin receptor binding activity in a rabbit antrum smooth muscle preparation and for stimulation of contractile activity in segments of rabbit duodenum. In vitro activity was directly correlated with motilin receptor binding affinity for all f Leu13]pMOT fragments examined. N-Terminal fragments of just over half the length of the native peptide are nearly equipotent as full-length motilin. These results suggest that the N-terminal segment, together with residues from the mid-portion of the molecule, constitutes the bioactive portion of pMOT. The C-terminal segment, in contrast, contributes little to receptor binding affinity or in vitro activity.
ISSN: 0196-9781
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Research in GastroIntestinal Disorders
× corresponding author
# (joint) last author

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