ITEM METADATA RECORD
Title: Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
Authors: Klionsky, Daniel J ×
Abeliovich, Hagai
Agostinis, Patrizia
Agrawal, Devendra K
Aliev, Gjumrakch
Askew, David S
Baba, Misuzu
Baehrecke, Eric H
Bahr, Ben A
Ballabio, Andrea
Bamber, Bruce A
Simon, Hans-Uwe
Simone, Cristiano
Simonsen, Anne
Smith, Mark A
Spanel-Borowski, Katharina
Srinivas, Vickram
Steeves, Meredith
Stenmark, Harald
Stromhaug, Per E
Brunk, Ulf T
Komatsu, Masaaki
Subauste, Carlos S
Sugimoto, Seiichiro
Sulzer, David
Suzuki, Toshihiko
Swanson, Michele S
Tabas, Ira
Takeshita, Fumihiko
Talbot, Nicholas J
Tallóczy, Zsolt
Tanaka, Keiji
Kominami, Eiki
Bursch, Wilfried
Tanaka, Kozo
Tanida, Isei
Taylor, Graham S
Taylor, J Paul
Terman, Alexei
Tettamanti, Gianluca
Thompson, Craig B
Thumm, Michael
Tolkovsky, Aviva M
Kondo, Seiji
Tooze, Sharon A
Camougrand, Nadine
Truant, Ray
Tumanovska, Lesya V
Uchiyama, Yasuo
Ueno, Takashi
Uzcátegui, Néstor L
van der Klei, Ida
Vaquero, Eva C
Vellai, Tibor
Kovács, Attila L
Vogel, Michael W
Wang, Hong-Gang
Cebollero, Eduardo
Webster, Paul
Wiley, John W
Xi, Zhijun
Xiao, Gutian
Yahalom, Joachim
Yang, Jin-Ming
Yap, George
Kroemer, Guido
Yin, Xiao-Ming
Yoshimori, Tamotsu
Yu, Li
Cecconi, Francesco
Yue, Zhenyu
Yuzaki, Michisuke
Zabirnyk, Olga
Zheng, Xiaoxiang
Zhu, Xiongwei
Deter, Russell L
Kuan, Chia-Yi
Chen, Yingyu
Chin, Lih-Shen
Choi, Augustine
Chu, Charleen T
Chung, Jongkyeong
Clarke, Peter G H
Clark, Robert S B
Clarke, Steven G
Clavé, Corinne
Cleveland, John L
Kumar, Rakesh
Codogno, Patrice
Colombo, María I
Coto-Montes, Ana
Cregg, James M
Cuervo, Ana Maria
Debnath, Jayanta
Demarchi, Francesca
Dennis, Patrick B
Dennis, Phillip A
Deretic, Vojo
Kundu, Mondira
Devenish, Rodney J
Di Sano, Federica
Dice, J Fred
Difiglia, Marian
Dinesh-Kumar, Savithramma
Distelhorst, Clark W
Djavaheri-Mergny, Mojgan
Dorsey, Frank C
Dröge, Wulf
Dron, Michel
Landry, Jacques
Dunn, William A
Duszenko, Michael
Eissa, N Tony
Elazar, Zvulun
Esclatine, Audrey
Eskelinen, Eeva-Liisa
Fésüs, László
Finley, Kim D
Fuentes, José M
Fueyo, Juan
Laporte, Marianne
Fujisaki, Kozo
Galliot, Brigitte
Gao, Fen-Biao
Gewirtz, David A
Gibson, Spencer B
Gohla, Antje
Goldberg, Alfred L
Gonzalez, Ramon
González-Estévez, Cristina
Gorski, Sharon
Bassham, Diane C
Gottlieb, Roberta A
Häussinger, Dieter
He, You-Wen
Heidenreich, Kim
Hill, Joseph A
Høyer-Hansen, Maria
Hu, Xun
Huang, Wei-Pang
Iwasaki, Akiko
Jäättelä, Marja
Le, Weidong
Jackson, William T
Jiang, Xuejun
Jin, Shengkan
Johansen, Terje
Jung, Jae U
Kadowaki, Motoni
Kang, Chanhee
Kelekar, Ameeta
Kessel, David H
Kiel, Jan A K W
Lei, Huan-Yao
Kim, Hong Pyo
Kimchi, Adi
Kinsella, Timothy J
Kiselyov, Kirill
Kitamoto, Katsuhiko
Knecht, Erwin
Lenardo, Michael J
Levine, Beth
Lieberman, Andrew
Lim, Kah-Leong
Lin, Fu-Cheng
Liou, Willisa
Liu, Leroy F
Lopez-Berestein, Gabriel
Bergamini, Ettore
López-Otín, Carlos
Lu, Bo
Macleod, Kay F
Malorni, Walter
Martinet, Wim
Matsuoka, Ken
Mautner, Josef
Meijer, Alfred J
Meléndez, Alicia
Michels, Paul
Bi, Xiaoning
Miotto, Giovanni
Mistiaen, Wilhelm P
Mizushima, Noboru
Mograbi, Baharia
Monastyrska, Iryna
Moore, Michael N
Moreira, Paula I
Moriyasu, Yuji
Motyl, Tomasz
Münz, Christian
Biard-Piechaczyk, Martine
Murphy, Leon O
Naqvi, Naweed I
Neufeld, Thomas P
Nishino, Ichizo
Nixon, Ralph A
Noda, Takeshi
Nürnberg, Bernd
Ogawa, Michinaga
Oleinick, Nancy L
Olsen, Laura J
Blum, Janice S
Ozpolat, Bulent
Paglin, Shoshana
Palmer, Glen E
Papassideri, Issidora
Parkes, Miles
Perlmutter, David H
Perry, George
Piacentini, Mauro
Pinkas-Kramarski, Ronit
Prescott, Mark
Bredesen, Dale E
Proikas-Cezanne, Tassula
Raben, Nina
Rami, Abdelhaq
Reggiori, Fulvio
Rohrer, Bärbel
Rubinsztein, David C
Ryan, Kevin M
Sadoshima, Junichi
Sakagami, Hiroshi
Sakai, Yasuyoshi
Brodsky, Jeffrey L
Sandri, Marco
Sasakawa, Chihiro
Sass, Miklós
Schneider, Claudio
Seglen, Per O
Seleverstov, Oleksandr
Settleman, Jeffrey
Shacka, John J
Shapiro, Irving M
Sibirny, Andrei
Brumell, John H
Silva-Zacarin, Elaine C M #
Issue Date: Mar-2008
Series Title: Autophagy vol:4 issue:2 pages:151-175
Abstract: Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
URI: 
ISSN: 1554-8627
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Laboratory of Cell Death Research & Therapy
× corresponding author
# (joint) last author

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