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Title: Baseline microbiota activity and initial bifidobacteria counts influence responses to prebiotic dosing in healthy subjects
Authors: De Preter, Vicky
Vanhoutte, Tom
Huys, Geert
Swings, Jean
Rutgeerts, Paul
Verbeke, Kristin # ×
Issue Date: 15-Mar-2008
Publisher: Blackwell Science
Series Title: Alimentary Pharmacology & Therapeutics vol:27 issue:6 pages:504-513
Abstract: Background: Dietary intervention with prebiotics can cause changes in the colonic microbiota and their metabolic activities. Aim: The present study investigated whether the response to prebiotic dosing is influenced by the baseline metabolic activity and bifidobacteria counts. Methods: The 4-wk effect of lactulose (10g b.i.d. (n=29)) and oligofructose-enriched inulin (OF-IN: 10g b.i.d. (n=19)) was evaluated in healthy human volunteers. Lactose-[(15)N, (15)N]-ureide was used to study the colonic NH(3)-metabolism. Urine (48-h) and faeces (72-h) were collected and analysed for p-cresol and (15)N-content by gas chromatography-mass spectrometry and isotope ratio mass spectrometer, respectively. Faecal bifidobacteria were quantified by real-time PCR. Results: After the 4-wk prebiotic administration period, the urinary excretion of p-cresol and (15)N was significantly decreased in both groups (p<0.05) corresponding with a significantly higher faecal excretion of (15)N (p<0.05). The decrease in urinary (15)N and p-cresol excretion significantly correlated with baseline (15)N and p-cresol levels (p<0.05), indicating that subjects with higher baseline levels showed a higher response to prebiotic dosing. Furthermore a significant correlation was seen between baseline bifidobacteria counts and the effect of prebiotic intake (p<0.05). Conclusions: The response to prebiotic dosing as indicated by the fate of NH(3), p-cresol and bifidobacteria is determined by the initial colonic conditions.
URI: 
ISSN: 0269-2813
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Research in GastroIntestinal Disorders
× corresponding author
# (joint) last author

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