Rapid protocol for electroporation of Clostridium perfringens
Jirásková, Alena × Vítek, Libor Fevery, Johan Ruml, Tomás Branny, Pavel #
Journal of microbiological methods vol:62 issue:1 pages:125-7
A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.