Title: Upregulation of Wilms' tumour gene 1 (WT1) in uterine sarcomas
Authors: Coosemans, An ×
Nik, S Amini
Caluwaerts, S
Lambin, Suzan
Verbist, G
Van Bree, R
Schelfhout, V
de Jonge, E
Dalle, I
Jacomen, G
Cassiman, Jean-Jacques
Moerman, Philippe
Vergote, Ignace
Amant, Frédéric #
Issue Date: Jul-2007
Publisher: Pergamon
Series Title: European Journal of Cancer vol:43 issue:10 pages:1630-1637
Abstract: AIM: Overexpression of Wilms' tumour gene (WT1) has been proven in several tumours. Previous research of our group on the cell cycle of uterine leiomyosarcoma (LMS) and carcinosarcoma (CS) suggested a possible role for WT1. We therefore intended to further explore the expression pattern of WT1 in uterine sarcomas. METHODS: 27 CS, 38 LMS, 15 endometrial stromal sarcomas (ESS) and seven undifferentiated sarcomas (US) were collected. WT1 expression was evaluated by immunohistochemistry (IHC) in 87 samples, by RT-PCR (m-RNA expression) in 23 random selected samples and by Western blotting in 12 samples, separating cytoplasmic and nuclear proteins. A pilot study to detect mutations (exons 7-10) was performed on eight samples. RESULTS: IHC showed WT1 positivity in 12/27 CS, 29/38 LMS, 7/15 ESS and 4/7 US. All-but-one sample had a positive RT-PCR. All Western blottings were positive with more cytoplasmic expression in 9/12 cases. No mutations were found. CONCLUSIONS: WT1 is overexpressed in uterine sarcomas. Since increased levels of mRNA determine the biological role, WT1 might contribute to uterine sarcoma tumour biology.
ISSN: 0959-8049
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Gynaecological Oncology
Section Woman - Miscellaneous (-)
Laboratory for Molecular Cell Biology (-)
Human Mutations and Polymorphisms Section (-)
Translational Cell & Tissue Research
Forensic Biomedical Sciences
× corresponding author
# (joint) last author

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