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Title: First preclinical evaluation of mono-[123I]iodohypericin as a necrosis-avid tracer agent
Authors: Ni, Yicheng ×
Huyghe, Dieter
Verbeke, Kristin
de Witte, Peter
Nuyts, Johan
Mortelmans, Luc
Chen, Feng
Marchal, Guy
Verbruggen, Alfons
Bormans, Guy M #
Issue Date: May-2006
Series Title: European Journal of Nuclear Medicine and Molecular Imaging vol:33 issue:5 pages:595-601
Abstract: PURPOSE: We have labelled hypericin, a polyphenolic polycyclic quinone found in St. John's wort (Hypericum perforatum), with( 123)I and evaluated mono-[(123)I]iodohypericin (MIH) as a potential necrosis-avid diagnostic tracer agent. METHODS: MIH was prepared by an electrophilic radioiodination method. The new tracer agent was evaluated in animal models of liver infarction in the rat and heart infarction in the rabbit using single-photon emission computed tomography (SPECT), triphenyltetrazolium chloride (TTC) histochemical staining, serial sectional autoradiography and microscopy, and radioactivity counting techniques. RESULTS: Using in vivo SPECT imaging, hepatic and cardiac infarctions were persistently visualised as well-defined hot spots over 48 h. Preferential uptake of the tracer agent in necrotic tissue was confirmed by perfect match of images from post-mortem TTC staining, autoradiography (ARX) and histology. Radioactivity concentration in infarcted tissues was over 10 times (liver; 3.51% ID/g in necrotic tissue vs 0.38% ID/g in normal tissue at 60 h p.i.) and over 6 times (myocardium; 0.36% ID/g in necrotic tissue vs 0.054% ID/g in normal tissue; ratios up to 18 for selected parts on ARX images) higher than in normal tissues. CONCLUSION: The results suggest that hypericin derivatives may serve as powerful necrosis-avid diagnostic agents for assessment of tissue viability.
URI: 
ISSN: 1619-7070
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Radiology
Translational Research in GastroIntestinal Disorders
Laboratory for Pharmaceutical Biology (-)
Nuclear Medicine & Molecular Imaging
Radiopharmacy
Theragnostic Laboratory (+)
× corresponding author
# (joint) last author

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