Title: The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H+-ATPase
Authors: Coccetti, P ×
Tisi, R
Martegani, E
Teixeira, LS
Brandao, RL
de Miranda Castro, I
Thevelein, Johan #
Issue Date: 1998
Series Title: Biochimica et Biophysica Acta. Molecular Cell Research vol:1405 issue:1-3 pages:147-154
Conference: date:Katholieke Univ Leuven, Mol Cell Biol Lab, B-3001 Louvain, Belgium; Univ Fed Ouro Preto, Escola Farm, Lab Bioquim & Fisiol Microorganismos, BR-3540000 Ouro Preto, MG, Brazil; Univ Milan, Fac Sci MF Nat 3, I-21100 Varese, Italy; Univ Milan, Dipartimento Fisiol & Biochim Gen, Sez Biochim Comparata, I-20133 Milan, Italy
Abstract: Addition of glucose to glucose-deprived cells of the yeast Saccharomyces cerevisiae triggers rapid turnover of phosphatidylinositol, phosphatidylinositol-phosphate and phosphatidylinositol 4,5-bisphosphate. Glucose stimulation of PI turnover was measured both as an increase in the specific ratio of P-32-labeling and as an increase in the level of diacylglycerol after addition of glucose. Glucose also causes rapid activation of plasma membrane H+-ATPase. We show that in a mutant lacking the PLCl encoded phospholipase C, both processes were strongly reduced. Compound 48/80, a known inhibitor of mammalian phospholipase C, inhibits both processes. However, activation of the plasma membrane H+-ATPase is only inhibited by concentrations of compound 48/80 that strongly inhibit phospholipid turnover. Growth was inhibited by even lower concentrations. Our data suggest that in yeast cells, glucose triggers through activation of the PLC1 gene product a signaling pathway initiated by phosphatidylinositol turnover and involved in activation of the plasma membrane H+-ATPase. (C) 1998 Elsevier Science B.V. All rights reserved.
ISSN: 0167-4889
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous (-)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science