Title: Fps1p controls the accumulation and release of the compatible solute glycerol in yeast osmoregulation
Authors: Tamas, MJ ×
Luyten, K
Sutherland, FCW
Hernandez, A
Albertyn, J
Valadi, H
Li, H
Prior, BA
Killan, SG
Ramos, J
Gustafsson, L
Thevelein, Johan
Hohmann, S #
Issue Date: 1999
Series Title: Molecular Microbiology vol:31 issue:4 pages:1087-1104
Conference: date:Katholieke Univ Leuven, Lab Mol Celbiol, B-3001 Leuven, Belgium; Univ Cordoba, Escuela Tecn Ingn Agron & Montes, Dept Microbiol, E-14080 Cordoba, Spain; Univ Orange Free State, Dept Microbiol & Biochem, ZA-9300 Bloemfontein, South Africa; Univ Gothenburg, Dept Cell & Mol Biol Microbiol, SE-40530 Gothenburg, Sweden
Abstract: The accumulation of compatible solutes, such as glycerol, in the yeast Saccharomyces cerevisiae, is a ubiquitous mechanism in cellular osmoregulation. Here, we demonstrate that yeast cells control glycerol accumulation in part via a regulated, Fps1 p-mediated export of glycerol. Fps1 p is a member of the MIP family of channel proteins most closely related to the bacterial glycerol facilitators. The protein is localized in the plasma membrane. The physiological role of Fps1 p appears to be glycerol export rather than uptake. Fps1 Delta mutants are sensitive to hypo-osmotic shock, demonstrating that osmolyte export is required for recovery from a sudden drop in external osmolarity, In wild-type cells, the glycerol transport rate is decreased by hyperosmotic shock and increased by hypo-osmotic shock on a subminute time scale. This regulation seems to be independent of the known yeast osmo-sensing HOG and PKC signalling pathways. Mutants lacking the unique hydrophilic N-terminal domain of Fps1p, or certain parts thereof, fail to reduce the glycerol transport rate after a hyperosmotic shock. Yeast cells carrying these constructs constitutively release glycerol end show a dominant hyperosmosensitivity, but compensate for glycerol loss after prolonged incubation by glycerol overproduction. Fps1p may be an example of a more widespread class of regulators of osmoadaptation, which control the cellular content and release of compatible solutes.
ISSN: 0950-382X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous (-)
× corresponding author
# (joint) last author

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