Author:

Keywords:

osmotic stress, high osmolarity glycerol pathway, mitogen-activated protein kinase, glycerol, transmembrane transport, ACTIVATED PROTEIN-KINASE, SACCHAROMYCES-CEREVISIAE, OSMOTIC-STRESS, RESPONSE PATHWAY, HEAT-SHOCK, GLYCEROL-3-PHOSPHATE DEHYDROGENASE, TRANSDUCTION PATHWAY, GENE-EXPRESSION, GROWTH DEFECT, BUDDING YEAST, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, Cell Biology, Blotting, Northern, Ethanol, Fungal Proteins, Glycerol, Membrane Proteins, Mitogen-Activated Protein Kinases, Osmolar Concentration, Osmotic Pressure, Phosphorylation, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, Sodium Chloride, 0304 Medicinal and Biomolecular Chemistry, 0601 Biochemistry and Cell Biology, 0603 Evolutionary Biology, 3101 Biochemistry and cell biology

Abstract:

The Saccharomyces cerevisiae HOG pathway controls responses to osmotic shock such as production of the osmolyte glycerol. Here we show that the HOG pathway can be stimulated by addition of glycerol. This stimulation was strongly diminished in cells expressing an unregulated Fps1p glycerol channel, presumably because glycerol rapidly equilibrated across the plasma membrane. Ethanol, which passes the plasma membrane readily and causes mater stress by disturbing the hydration of biomolecules, did not activate the HOG pathway. These observations suggest that stimulation of the HOG pathway is mediated by a turgor change and not by water stress per se. (C) 2000 Federation of European Biochemical Societies.