ITEM METADATA RECORD
Title: GROWTH-RELATED EXPRESSION OF RIBOSOMAL-PROTEIN GENES IN SACCHAROMYCES-CEREVISIAE
Authors: KRAAKMAN, LS ×
GRIFFIOEN, G
ZERP, S
GROENEVELD, P
Thevelein, Johan
MAGER, WH
PLANTA, RJ #
Issue Date: 1993
Publisher: Springer-Verlag Heidelberg
Series Title: Molecular & General Genetics vol:239 issue:1-2 pages:196-204
Conference: date:FREE UNIV AMSTERDAM,DEPT BIOCHEM & MOLEC BIOL,1007 MC AMSTERDAM,NETHERLANDS; FREE UNIV AMSTERDAM,DEPT MICROBIOL,1007 MC AMSTERDAM,NETHERLANDS; CATHOLIC UNIV LEUVEN,MOLEC CELL BIOL LAB,B-3030 HEVERLEE,BELGIUM
Abstract: The rate of ribosomal protein gene (rp-gene) transcription in yeast is accurately adjusted to the cellular requirement for ribosomes under various growth conditions. However, the molecular mechanisms underlying this co-ordinated transcriptional control have not yet been elucidated. Transcriptional activation of rp-genes is mediated through two different multifunctional transacting factors, ABF1 and RAP1. In this report, we demonstrate that changes in cellular rp-mRNA levels during varying growth conditions are not parallelled by changes in the in vitro binding capacity of ABF1 or RAP1 for their cognate sequences. In addition, the nutritional upshift response of rp-genes observed after addition of glucose to a culture growing on a non-fermentative carbon source turns out not to be the result of increased expression of the ABF1 and RAP1 genes or of elevated DNA-binding activity of these factors. Therefore, growth rate-dependent transcription regulation of rp-genes is most probably not mediated by changes in the efficiency of binding of ABF1 and RAP1 to the upstream activation sites of these genes, but rather through other alterations in the efficiency of transcription activation. Furthermore, we tested the possibility that cAMP may play a role in elevating rp-gene expression during a nutritional shift-up. We found that the nutritional upshift response occurs normally in several mutants defective in cAMP metabolism.
ISSN: 0026-8925
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous
× corresponding author
# (joint) last author

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