Title: Permeation of hypericin in spheroids composed of different grade transitional cell carcinoma cell lines and normal human urothelial cells
Authors: Huygens, Ann
Kamuhabwa, Appolinary R
Roskams, Tania
Van Cleynenbreugel, Ben
Van Poppel, Hendrik
de Witte, Peter # ×
Issue Date: Jul-2005
Series Title: Journal of Urology vol:174 issue:1 pages:69-72
Abstract: PURPOSE: We investigated the importance of E-cadherin expression on the selective accumulation of hypericin in superficial bladder cancer after intravesical instillation. MATERIALS AND METHODS: Spheroids obtained from a panel of 3 transitional cell carcinoma cell lines, namely J-82, RT-4 (American Type Culture Collection, Manassas, Virginia) and RT-112 (German Collection of Micro-organisms and Cell Cultures, Braunschweig, Germany), and normal human urothelial (NHU) cells were incubated with hypericin. Accumulation was examined with fluorescence microscopy. Immunohistochemical staining was used to assess E-cadherin expression.
RESULTS: Immunohistochemical staining showed E-cadherin expression in NHU (++), RT-112 (+) and RT-4 (+) spheroids, whereas E-cadherin expression was absent in J-82 spheroids. The highest intraspheroidal hypericin accumulation was observed in transitional cell carcinoma spheroids, whereas limited permeation was seen in NHU spheroids. Taken together the data point to an inverse relationship between E-cadherin expression and the permeation of hypericin throughout a 3-dimensional cellular matrix.
CONCLUSIONS: Loss of E-cadherin expression correlates with loss of intercellular adhesion, tight junction formation and enhanced paracellular transport. The data show that E-cadherin hampers the permeation of hypericin in spheroids and the loss of intercellular adhesion, present in superficial bladder cancer lesions, can be associated with enhanced hypericin permeation. Therefore, E-cadherin expression seems to have a pivotal role in the selective uptake of hypericin after intravesical instillation in human bladders.
ISSN: 0022-5347
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Cell & Tissue Research
Urology Section (-)
Laboratory for Pharmaceutical Biology (-)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science